Isolation, Characterization, and Optimization of Keratinase from Bacillus cereus BRAW_KM

Indonesia possesses tremendous marine resources. Therefore, their products are appropriate for exploration. In the prior study, bacteria generating keratinase enzyme have isolated from local fish market trash. The may hydrolyze keratin on skin. Surrounding parameters, such as temperature, pH, and incubation duration, factors affecting activity of enzyme. This study aims to isolate characterize keratinase, optimize its production. Bacillus cereus BRAW_KM was main material utilized in this research. First, keratinolytic bacterium investigated properties using native polyacrylamide gel electrophoresis (PAGE) sodium dodecyl sulfate (SDS)-PAGE. Then, ideal conditions synthesis were adjusted by time activity. Of 10 isolations discovered, one shows potential a bacterium, which tends behave like sp. molecular weights 130 kDa 95 kDa. optimum B. at 29 °C, pH 9, 90 minutes incubation.